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Á¦¸ñ¿¡¼­ ³»¿ë¿¡¼­ À̸§¿¡¼­ ÀÚ¿¬¾î°Ë»ö
ºÎÁ¤¾î °ø¹é±¸ºÐ °Ë»ö ¿¬»êÀÚ and or
°Ë»öµÈ ºÐ·ù ¸®½ºÆ® (1°³ÀÇ ºÐ·ù)
°Ë»öµÈ °Ô½Ã¹° ¸®½ºÆ® (15°³ÀÇ °Ô½Ã¹° 1/1ÆäÀÌÁö)
  • Gene cloning & transformation ÇѱÛÆÄÀÏ-_-; [ÇöÀçâ]

  • selection) ÀÌ °¡´ÉÇÏ´Ù. Vector¿¡ Á¸ÀçÇÏ´Â Ç×»ýÁ¦ ³»¼º À¯ÀüÀÚ¸¦ ÀÌ¿ëÇϸé DNA°¡ ¼¼Æ÷ ³»·Î ÁÖÀԵǾú´ÂÁö ¾Æ ´ÑÁö¸¦ ÆǺ°ÇÒ ¼ö Àִµ¥ ¼¼Æ÷ ¼ÓÀ¸·Î DNA°¡ µé¾î°£ ¼¼Æ÷¸¸À» ¼±º°ÇÏ´Â °ÍÀ» selection À̶ó°í ÇÑ´Ù. 3. Multiple cloning site (MCS)¸¦ ÀÌ¿ëÇÒ ¼ö ÀÖ°Ô ÇØ ÁØ´Ù. vectorÀÇ ÀÏÁ¤ºÎÀ§¿¡...
    2005³â04¿ù05ÀÏ 16½Ã16ºÐ32ÃÊ by ±è¼º°ï
  • Cell Signaling [ÇöÀçâ]

  • selectively against cancer cells with less toxicity toward normal cells than that of conventional chemotherapeutic agents. 3. Ras-targented drugs: farnesyl transferase inhibitor ¡ß display substantial selectivity in their action against tumor cells expressing oncogenic Ras protein ...
    2005³â01¿ù24ÀÏ 19½Ã28ºÐ01ÃÊ by ¿À¿µ¹Î
  • LARGE-SCALE PLASMID PREP [ÇöÀçâ]

  • selection shaking at 37oC. When OD600 = 0.8 you may add chloroamphenicol to 125ug/ml. Let it go overnight. B. Spin down the cultures in sterile bottles at 5000rpm for 10 min at 4oC. Make sure the seals on the bottle are properly seated and the bottles are balanced within 0.1g. Carefu...
    2004³â04¿ù30ÀÏ 10½Ã52ºÐ25ÃÊ by ¿À¿µ¹Î
  • LARGE-SCALE PLASMID PREP [ÇöÀçâ]

  • selection shaking at 37oC. When OD600 = 0.8 you may add chloroamphenicol to 125ug/ml. Let it go overnight. B. Spin down the cultures in sterile bottles at 5000rpm for 10 min at 4oC. Make sure the seals on the bottle are properly seated and the bottles are balanced within 0.1g. Carefu...
    2004³â04¿ù30ÀÏ 10½Ã52ºÐ25ÃÊ by ¿À¿µ¹Î
  • Chapter 7. Cell [ÇöÀçâ]

  • selective barrier·Î¼­ cellÀÇ Àüü volume¿¡ ´Ã·Á´Â ±â´ÉÀ» ÇÑ´Ù. </P> <P><IMG src="http://khugene.com/v2/geneschool/data_image/plasma_membrane.jpg" border=0> </P> <P> </P> <P>¢Ñ <STRONG>compartmentalization </STRONG></P> ...
    2004³â05¿ù01ÀÏ 12½Ã51ºÐ47ÃÊ by ¿À¿µ¹Î
  • Chapter 5. The structure and function of macromolecles [ÇöÀçâ]

  • selective acceleration ex. digetive enzyme ¡à Polypeptide - amino acids : 20 Á¾·ù°¡ ÀÖ´Ù. <img src=http://khugene.com/v2/geneschool/data_image/amino_acid.jpg border=0 > <img src=http://khugene.com/v2/geneschool/data_image/20_amino_acids.jpg border=0 > - condensation of...
    2004³â04¿ù29ÀÏ 22½Ã45ºÐ17ÃÊ by ¿À¿µ¹Î
  • »ý¹°ÀÇ Æ¯Â¡ [ÇöÀçâ]

  • selection¿¡ ÀÇÇØ ¿ì¼ºÀÇ ÇüÁúÀº ³²±â·ÁÇÏ°í ¿­¼ºÀº µµÅµǷÁÇÑ´Ù. d. ¼ºÀåÇÏ°í ¹ß´ÞÇÑ´Ù. (¹«°Ô, ºÎÇÇ, Å©±âÀÇ º¯È­ ; ¼ºÀåÀûÀÎ Ãø¸é / ³»ºÎÀÚ±Ø, È£¸£¸ó ºÐºñ ; ¹ß´ÞÀûÀÎ Ãø¸é , ¼öÁ¤¶õ -&gt; ¹ß»ý -&gt; ºÐÈ­ -&gt; °³Ã¼ ; ¹ø½ÄÀÇ °¡´É¿©ºÎ) e. Àڱؿ¡ ´ëÇÏ¿© ¹ÝÀÀÇÑ´Ù. ; ÁÖ¸° ¹è¸¦ ä¿ì±â À§ÇØ ÀÌ...
    2004³â03¿ù29ÀÏ 01½Ã07ºÐ32ÃÊ by ¿À¿µ¹Î
  • Gene tagging (insertional mutagenesis) [ÇöÀçâ]

  • selection¿¡ ÀÇÇØ ´ëÇ×À» Á» ´õ È¿°úÀûÀ¸·Î Àß ÇÏ´Â °³Ã¼¸¸ÀÌ »ì¾Æ³²°Ô µÈ´Ù. ¡æ ±×·¯¹Ç·Î DNA°¡ ´õ¿í ´õ º¹ÀâÇÏ°Ô µÈ´Ù. d. Entrapment tagging : promoterless reporter promoter¿Í gene »çÀÌÀÇ °ø°£¿¡ known DNA sequence(-- Reporter gene / Ter --)°¡ insert µÇ´Â °ÍÀ» trapÀ̶ó ÇÑ´Ù. inset µÇ´Â DNA seq...
    2004³â04¿ù12ÀÏ 01½Ã54ºÐ02ÃÊ by ¿À¿µ¹Î
  • chap.2 Vehicles for gene cloning &#58; plasmid and bac [ÇöÀçâ]

  • selectable marker * ¸ðµç plasmidsms origin of replicationÀ» ÇÏ´Â Çϳª ÀÌ»óÀÇ DNA sequence¸¦ °¡Áö°í ÀÖ´Ù. --&gt; main bacterial chromosome¿¡ ²Ï µ¶¸³ÀûÀ¸·Î cell¿¡¼­ multiply°¡ °¡´É. * ¾î¶² plasmid´Â bacterial chromosome ¾È¿¡ insertingµÇ¾î replicate°¡ °¡´ÉÇÏ´Ù ex) integrative plasmid(=episom...
    2003³â01¿ù11ÀÏ 13½Ã16ºÐ23ÃÊ by ¿À¿µ¹Î
  • chap.5 Preservation of animal cell lines [ÇöÀçâ]

  • selectÇÑ´Ù. ¨ê ÁÖ±âÀûÀ¸·Î working stockÀ» replenish ÇÒ ¼ö ÀÖ´Â cell lineÀÇ seed stockÀ» º¸°üÇÑ´Ù. 5.2. Variation and instablility in cell lines 5.2.1. Culture conditions cell line maintainingÀ» À§ÇØ optimum conditionÀÌ establishµÇ¸é, °¡´ÉÇÑ ±×´ë·Î À¯ÁöÇÑ´Ù. cell culture mediumÀÇ constitue...
    2003³â01¿ù10ÀÏ 11½Ã16ºÐ35ÃÊ by ¿À¿µ¹Î
  • chap.3 Animal cell culture media [ÇöÀçâ]

  • selective inhibitor, bacterial endotoxin, lipid, polyamin oxidase¸¦ Æ÷ÇÔÇÏ°í ÀÖ´Ù. d. specific growth factor´Â ¾î¶² cellÀ» ÀÚ¶ó°Ô Çϴµ¥ Àû´ç·®ÀÌ ÀÖ¾î¾ß ÇÑ´Ù. --&gt; purified growth factor¸¦ °è¼Ó ÷°¡ÇØ ÁÖ¾î¾ß ÇÑ´Ù. e. ¸Å¿ì expensiveÇÏ°í °ø±Þ¿¡ Á¦ÇÑÀÌ ÀÖ´Ù. cell type¿¡ µû¶ó 5 ~ 20 % »çÀÌÀÇ...
    2002³â12¿ù22ÀÏ 19½Ã08ºÐ12ÃÊ by ¿À¿µ¹Î
  • chap.2 Animal cell cultureÀÇ Áß¿ä±â¼ú [ÇöÀçâ]

  • selecting cell lines °ÅÀÇ ¸ðµç primary explant cultures´Â ¸¹Àº heterogeneous populations of cells·Î ±¸¼ºµÇ¾î ÀÖ´Ù. slow growing cells´Â faster growing cell¿¡ ÀÇÇØ ºü¸£°Ô overgrowth ÇÑ´Ù. specific cell typeÀº single cell typeÀÇ pure cell lines¸¦ »ý»êÇϱâ À§ÇØ cloningÀ̳ª physical separation te...
    2002³â12¿ù17ÀÏ 12½Ã13ºÐ59ÃÊ by ¿À¿µ¹Î
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